Journal: Regenerative Therapy

Article Title: Platelet rich plasma promotes cell migration of adipose-derived stem cells by up-regulation of focal adhesion kinase and paxillin

doi: 10.1016/j.reth.2025.05.004

Figure Lengend Snippet: PRP increased phosphorylated FAK, FAK, phosphorylated paxillin, paxillin, and vinculin expression in ADSCs . ADSCs were subjected to 24 h of treatment with 0.5 %, 1 %, or 2 % PRP. (A) Western blot analysis of phosphorylated FAK, FAK, phosphorylated paxillin, paxillin, and vinculin. (B) Phosphorylated FAK, FAK, paxillin, phosphorylated paxillin, and vinculin band intensities. ∗p < 0.05 compared with the control. p- FAK means phosphorylated FAK, p -Paxillin means phosphorylated paxillin.

Article Snippet: After blocking with 5 % BSA in Tris-buffered saline with 0.1 % Tween-20 (TBST) for 1 h, the membranes were incubated in primary antibodies, e.g. anti- p -FAK, anti-FAK, anti-vinculin, anti-paxillin (3281, 3285, 13901, 2542, Cell Signaling Technology), anti-phosphorylated paxillin (44–722, Thermo Fisher Scientific) and anti-Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (60004-1, Proteintech Group, Inc.) for 2 h. After washing in TBST, the membranes were incubated in horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology) for 1 h. The signal was examined by using Luminata Crescendo Western HRP substrate (Merck Millipore, Darmstadt, Germany).

Techniques: Expressing, Western Blot, Control

Journal: Regenerative Therapy

Article Title: Platelet rich plasma promotes cell migration of adipose-derived stem cells by up-regulation of focal adhesion kinase and paxillin

doi: 10.1016/j.reth.2025.05.004

Figure Lengend Snippet: ADSCs were treated with FAK, paxillin, or control siRNA for migration analysis . ADSCs were transfected with FAK siRNA, paxillin siRNA or control siRNA. (A) Protein expression levels of FAK and paxillin were assessed by Western blot analysis. (B) Cell migration ability was evaluated using a transwell filter migration assay. (C) Quantification of relative cell migration rates. Scale bars: 50 μm ∗p < 0.05 vs. control group; #p < 0.05 vs. control siRNA group. NC si, FAK si, and Pax si represent the control, FAK, and paxillin siRNA groups, respectively.

Article Snippet: After blocking with 5 % BSA in Tris-buffered saline with 0.1 % Tween-20 (TBST) for 1 h, the membranes were incubated in primary antibodies, e.g. anti- p -FAK, anti-FAK, anti-vinculin, anti-paxillin (3281, 3285, 13901, 2542, Cell Signaling Technology), anti-phosphorylated paxillin (44–722, Thermo Fisher Scientific) and anti-Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (60004-1, Proteintech Group, Inc.) for 2 h. After washing in TBST, the membranes were incubated in horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology) for 1 h. The signal was examined by using Luminata Crescendo Western HRP substrate (Merck Millipore, Darmstadt, Germany).

Techniques: Control, Migration, Transfection, Expressing, Western Blot